Description
Description: This assay employs a two-site sandwich ELISA to quantitate GABRB2 in samples. An antibody specific for GABRB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GABRB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GABRB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GABRB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: GABRB2 is a member of the GABA-A receptor gene family of heteromeric pentameric ligand-gated ion channels through which GABA, the major inhibitory neurotransmitter in the mammalian brain, acts. GABA-A receptors are the site of action of a number of important pharmacologic agents including barbiturates, benzodiazepines, and ethanol. Both GABA-A receptors and the GABA synthetic enzyme glutamic acid decarboxylase are expressed in pulmonary epithelial cells. Activation of GABA-A receptors depolarized these cells. The expression of glutamic acid decarboxylases GAD65 and GAD67 in the cytosol and GABA-A receptors in the apical membranes of airway epithelial cells increased markedly when mice were sensitized and then challenged with ovalbumin, an approach for inducing allergic asthmatic reactions